Figure 1.

Ligation of DNA target by various adapters. (A) Sequences of the three adapters with various lengths. (B) DNA target (Ta/Tb) is ligated by adapters with 5–7 bp long duplex parts. Lane 1, the Ta/Tb duplex (38 bp) with a 5-nt overhang; lane 2, 4, 6, Ta/Tb is ligated by phosphorylated adapters; lane 3, 5, 7, Ta/Tb is ligated by un-phosphorylated adapters. [target DNA] = 1 μmol/L, [adapter] = 1 μmol/L, 2.5 U T4 DNA ligase at 25 °C for 2 h. (C) Cyclization products are analyzed by qPCR. Ligation reactions: [558-bp target] = 300 amol (10⁷ copies/μL), [adapter] = 10 nmol/L, 2.5 U T4 DNA Ligase at 25 °C for 2 h; qPCR reactions: [primers] = 0.4 μmol/L, 1 μL ligation products.