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. 2022 Jan 20;11(3):273. doi: 10.3390/foods11030273

Figure 6.

Figure 6

Effect of PCR-C of different storage periods, Fresh and NF-10 on t-BHP-induced ROS (A), and MDA (B) generation and reduced SOD (C) and GSH (D) depletion. (A) HepG2 cells were pretreated with 50 µg/mL NF-10, Fresh, PCR-C01, PCR-C03, PCR-C05, and PCR-C10 for 24 h, then incubated with DCFH-DA for 30 min at 37 °C, then with t-BHP (300 µM) for 1.5 h. ROS levels were determined by a microplate fluorimeter. (B–D) HepG2 cells were pretreated with 50 µg/mL NF-10, Fresh, PCR-C01, PCR-C03, PCR-C05, and PCR-C10 for 24h, and then incubated with t-BHP (300 µM) for 3 h. MDA, SOD activity, and GSH content were determined using MDA, T-SOD and GSH assay kit, respectively. Results are presented as mean ± SD, n = 6, ns means no significant difference. ** p < 0.01, *** p< 0.001 vs. control group.