Table 1.
Summary of the protocols of immunohistochemistry used in this study.
| Antibody | Supplier (Catalogue Number) | Host | Antigen Retrieval Treatment | Dilution | Reaction Time of Primary Antibody | Antibody Type | Secondary Antibody | Positive Control |
|---|---|---|---|---|---|---|---|---|
| Ki-67 | Agilent technologies, US (IR626) | Mouse | PT Link (97 ℃, 20 min), Target Retrieval Solution High PH | Ready to use | 20 min, room temperature | MIB-1 | EnVision FLEX | Epithelial cell |
| SSTR2 | Abcam, England (ab134152) | Rabbit | AC (121 ℃, 5 min), pH 6.0 | 1:2000 | 4 ℃, overnight | UMB1 | Histofine Kit | Islet of langerhans |
| Chromogranin A | Agilent technologies, US (A0430) | Rabbit | Microwave (210 W, 15 min), pH 6.0 | 1:1500 | 4 ℃, overnight | polyclonal | Histofine Kit | Islet of langerhans |