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. 2022 Feb 3;11(3):532. doi: 10.3390/cells11030532

Figure 1.

Figure 1

GILZ deficiency causes a greater inflammatory response to LPS-induced Acute Lung Injury. C57BL/6 WT or GILZ−/− mice were stimulated with LPS (1 µg, i.n.) and euthanized 24 h later (schematic strategy in (A)). BAL was harvested to quantify the number of total leukocytes (B), neutrophils (C) and macrophages (D). Levels of the cytokines TNF-α (E) and IL-6 (F) and chemokines CXCL-1 (G) and CXCL-2 (H) were measured by ELISA in BAL fluid. Graph (I) shows the percentage of efferocytosis by morphological counting of cytospin slides stained with May-Grunwald-Giemsa. In (J), arrows indicate apoptotic neutrophils inside macrophages. Magnification 100×. Data are mean ± SEM of N = 6 animals per group. * p < 0.05, ** p < 0.01, *** p < 0.001 or **** p < 0.0001 when compared to the saline group; or as indicated: # p < 0.05, ## p < 0.01 ### p < 0.001 or #### p < 0.0001 when comparing LPS-challenged GILZ−/− to WT mice, by 2-way ANOVA.