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. 2022 Feb 3;11(3):532. doi: 10.3390/cells11030532

Figure 3.

Figure 3

TAT-GILZ treatment during LPS-induced ALI modulates neutrophilic inflammation and increases efferocytosis indexes. C57BL/6 WT mice were stimulated with LPS (1 µg, i.n.), treated with TAT (0.1 mg/kg, i.p.) or TAT-GILZ (0.2 mg/kg, i.p.) 12 h p.i., and euthanized 24 h later (schematic protocol in (A)). BAL was harvested to quantify number of total leukocytes (B), neutrophils (C) and macrophages (D). Levels of the cytokines TNF-α (E) and IL-6 (F) were measured by ELISA in BAL fluid. Graph (G) shows the percentage of efferocytosis by morphological counting of cytospin slides stained with May-Grunwald-Giemsa. In (H), arrows indicate apoptotic neutrophils inside macrophages. Magnification 100×. Vehicle group received DMSO 0.6%. Data are mean ± SEM of N = 4–5 animals per group. * p < 0.05, ** p < 0.01, *** p < 0.001 or **** p < 0.0001 when compared to saline instilled groups; or as indicated: # p < 0.05, ## p < 0.01 or p = 0.07 when comparing TAT-GILZ-treated LPS-challenged mice to vehicle or TAT groups, by 1-way ANOVA.