Table 6.
The IC50 values determined in antioxidant tests.
| Sample | IC50 | ||
|---|---|---|---|
| DPPH [μg/mL] | ABTS [μg/mL] | CHEL [μg/mL] | |
| CHi | 21.73 ± 0.13 e,f,g,h,i,k,l | 4.14 ± 0.10 e,f,g,h,i,j,k,l | 3.54 ± 0.15 f |
| CHi-1 | 32.37 ± 0.19 a,e,f,g,h,i,k,l | 5.48 ± 0.12 a,e,f,g,h,i,j,k,l | 182.67 ± 4.19 a,e,f,g,h,i,m |
| CHi-2 | 8.83 ± 0.10 a,b,e,f,g,h,i,j,k,l | 1.68 ± 0.03 a,b,e,f,g,h,i,k,l | 2.94 ± 0.03 b,f |
| CHi-3 | 13.69 ± 0.14 a,b,c,e,f,g,h,i,j,k,l | 2.74 ± 0.11 a,b,c,e,f,g,h,i,j,k,l | 4.04 ± 0.16 b,f |
| CHi-4 | 5.40 ± 0.10 a,b,c,d,e,f,g,h,i,j,k,l | 0.90 ± 0.02 a,b,c,d,e,f,i,j,k,l | 1.19 ± 0.05 b,f,i,m |
| CHs | 10.16 ± 0.02 j,k,l | 1.92 ± 0.13 j,l | 1.73 ± 0.10 j,m |
| CHs-1 | 18.15 ± 0.13 e,j,k,l | 1.53 ± 0.09 e,j | 76.50 ± 1.45 e,j,m |
| CHs-2 | 4.15 ± 0.05 e,f,j,k,l | 0.80 ± 0.02 e,f,j,k,l | 1.10 ± 0.03 f,j,m |
| CHs-3 | 3.43 ± 0.02 e,f,g,j,k | 0.68 ± 0.05 e,f,j,k,l | 1.01 ± 0.01 f,j,m |
| CHs-4 | 2.08 ± 0.03 e,f,g,h,k,l | 0.37 ± 0.01 e,f,g,h,j,k,l | 0.50 ± 0.01 f,j,m |
| quercetin | 2.05 ± 0.10 | 3.27 ± 0.23 | 6.24 ± 0.19 |
| AA | 4.75 ± 0.16 | 1.73 ± 0.09 | nt |
| Trolox | 3.68 ± 0.09 | 1.31 ± 0.05 | nt |
| Na2EDTA*2H2O | nt | nt | 4.15 ± 0.10 |
Data were expressed as mean ± SD, n = 3. AA—ascorbic acid; Na2EDTA*2H2O—ethylenediaminetetraacetic acid, disodium dihydrate; nt—not tested; CHi—crude extract of C. hissaricus, CHs—crude extract of C. hsingshangensis, CHi-1/CHs-1—water fraction, CHi-2/CHs-2—diethyl ether fraction, CHi-3/CHs-3—butanol fraction, CHi-4/CHs-4—ethyl acetate fraction; ABTS—2,2′-azino-bis-(3-ethyl-benzothiazole-6-sulfonic acid); CHEL—metal chelating activity. Statistical analysis: a—significantly different results compared to CHi; b—significantly different results compared to CHi-1; c—significantly different results compared to CHi-2; d—significantly different results compared to CHi-3; e—significantly different results compared to CHs; f—significantly different results compared to CHs-1; g—significantly different results compared to CHs-2; h—significantly different results compared to CHs-3; i—significantly different results compared to CHs-4; j—significantly different results compared to quercetin; k—significantly different results compared to AA; l—significantly different results compared to Trolox; m—significantly different results compared to Na2EDTA*2H2O; One-Way ANOVA test, followed by a Tukey’s multiple comparison test, p < 0.05.