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. 2022 Feb 5;11(3):560. doi: 10.3390/cells11030560

Figure 2.

Figure 2

PC12 sub-cell line obtained after 20 hypoxia–reoxygenation cycles revealed enhanced proliferative and pro-metastatic behavior under normoxic conditions compared with control cells. (A) PC12 Z20 cells showed an increased cell viability compared with PC12 Z20 control cells. Twelve independent experiments (n = 36). (B) The growth rate of PC12 Z20 cells was significantly higher under normoxic conditions and to some extent also under hypoxic conditions compared with control cells. A total of six independent experiments (n = 12). (C) Clonogenic survival assays revealed an enhanced plating efficiency of PC12 Z20 cells. A total of three independent experiments (n = 3). The (D) migration capacity of the PC12 Z20 cells was increased, while the (E) invasion capacity was not affected. From five to six independent experiments (n = 32–36). PC12 Z20 cells showed a diminished adhesion to (F) collagen and in trend to (G) laminin compared with the control cells. A total of four independent experiments (n = 16). Mean ± SEM; unpaired t-test for the comparison of two groups or Anova and Bonferroni post hoc test for more than two groups; comparison vs. PC12 Z20 control * p < 0.05, ** p < 0.001; comparison vs. normoxic conditions ## p < 0.001.