Figure 5.

Transglycosylation product analysis of recombinant TsBgl1. (A) HPLC chromatograms of transglycosylation products and formulas of the disaccharides synthetized by TsBgl1. Lam, laminaribiose (Glc-β-1,3-Glc). Cel, cellobiose (Glc-β-1,4-Glc). Sop, sophorose (Glc-β-1,2-Glc). Gen, gentiobiose (Glc-β-1,6-Glc). The formulas of the disaccharides were shown in the boat conformation, and the position of the linked hydroxyl group from the non-reducing end to the reducing end were annotated by arrow. (B) Time course of TsBgl1 catalyzed transglycosylation. The reaction was performed by adding 500 U·g glucose⁻¹ TsBgl1 and using 1000 g·L⁻¹ glucose as substrates at 80 °C and pH 6.0.