Table 2.
Antibody description and validation.
| Antibody Information | |||||
|---|---|---|---|---|---|
| Antibody | Manufacturer, Catalogue Number, Lot Number |
Peptide/ Protein Target |
Antigen Sequence |
Species Raised, Monoclonal, Polyclonal |
Dilution |
| Anti-AKR1B1 | Abcam, Cambridge, UK, ab62795, GR64780-2 |
Aldo-keto reductase family 1 member B1 | Aa 300 to the C-terminus (conjugated to keyhole limpet hemocyanin) | Polyclonal rabbit antibody | 1:200 |
| Anti-AKR1B10 | Abcam, Cambridge, UK, ab96417, GR13314-31 |
Aldo-keto reductase family 1 member B10 | Fragment corresponding to aa 1–286 | Polyclonal rabbit antibody | 1:200 |
| Antibody Validation | |||||
| Published validation by our research team [28]. | |||||
|
Current Validation Positive controls for AKR1B1: Kupffer cells, lymphocytes, endometrioid endometrial cancer cells. Positive controls for AKR1B10: hepatocytes, ductal liver epithelium, lymphocytes, endometrioid endometrial cancer cells. Negative controls for AKR1B1: hepatocytes, fibrous tissue. Negative controls for AKR1B10: fibrous tissue. | |||||
AKR1B1: aldo-keto reductase family 1 member B1; AKR1B10: aldo-keto reductase family 1 member B10.