Figure 7.

Chromatin condensation was assessed using the Hoechst 33342 assay after bisphenol A (BPA), diethylstilbestrol (DES, 4-tert-amylphenol (AP), 4-heptylphenol (HP), triclosan (T), propylparaben (PrP), benzyl butyl phthalate (BBP), DEHP and 3-benzylidene camphor (3BC) incubation for 72 h in JEG-Tox cells. The cells were preincubated with either PBS (solid bars) or with BBG at 25 µM (hatched bars) for 15 min. The solutions were removed and the cells were incubated with diethylstilbestrol, 4-heptylphenol, butyl benzyl phthalate (BBP) and DEHP. For clarity’s sake, EDCs abbreviations are used in Figure 4, Figure 6 and Figure 7. The significance thresholds were **** p < 0.0001 and * p < 0.1 compared with the control and # p < 0.1.