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. 2022 Jan 18;11(3):315. doi: 10.3390/cells11030315

Figure 2.

Figure 2

GFP-p1380N-ttLbCas12a:LOBP-directed indels in Pummelo leaf via Xcc-facilitated agroinfiltration. (a) Pre-treated with XccgumC:Tn5, Pummelo leaf was agroinfiltrated with Agrobacterium cells harboring GFP-p1380N-ttLbCas12a:LOBP. After four days, GFP fluorescence was observed. Agrobacterium cells harboring p1380-AtHSP70BP-GUSin was used as a negative control. (b) ttLbCas12a-directed modification of LOBP. The targeted sequence was shown in blue, and the mutations were shown in purple. (c) The representative chromatograms of EBEPthA4-TII LOBP and its mutations. The targeted sequence within LOBP was underlined by black lines, and the mutant site was indicated with an arrow. EBEpthA4-TII LOBP was highlighted by red rectangles.