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. 2022 Jan 18;11(3):315. doi: 10.3390/cells11030315

Figure 4.

Figure 4

Detection of genome editing of GFP-p1380N-ttLbCas12a:LOBP-transformed Pummelo by direct sequencing of PCR products. The chromatograms of direct sequencing of PCR products. Primers LOBP2 and LOBP5 were used to amplify LOBP from wild type and transgenic Pummelo. Direct sequencing primer was LOB4. The mutation site or the beginning sites of double/multiple peaks were shown by arrows. The targeted sequence was underlined by black lines and EBEPthA4-TII LOBP was highlighted by red rectangles.