Figure 5.
Homozygous line #Pumtt2. (a) Direct sequencing of PCR product of #Pumtt2. Upper: The chromatograms of three different leaves from #Pumtt2 were shown. The chromatograms are consistent with one another, which verified #Pumtt2 to be homozygous. EBEPthA4-LOBP was highlighted by red rectangles. The targeted sequence was underlined by black lines, and the mutation sites were indicated with arrows. The chromatogram of wild type Pummelo plant was included for comparison purpose. Lower: The targeted sequence is shown in blue, and the mutations are shown in purple. (b) Sanger sequencing results of #Pumtt2. Among 10 colonies sequenced, all of them are taaacccctt deletion. Type II LOBP in #Pum1. A part of LOBP sequences and its chromatogram are shown.