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. 2022 Jan 30;11(3):485. doi: 10.3390/cells11030485

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Evaluation of focal ischemic brain lesion by photothrombosis. (A) The laser speckle imagining system used for local blood perfusion evaluation; (B) The laser equipment used induce focal ischemic lesion by photothrombosis; (C) Baseline local blood perfusion before stroke induction; (D) Local perfusion after stroke induction; (E,F) The graphic representation of blood perfusion before and after stroke induction respectively; (G,H) Visualization of the animal’s skull with craniectomy before and after stroke induction, respectively; (I) Evaluation of stroke induction by TTC; and (J) Box plot of local blood perfusion change in the G2–G5 experimental groups. G2: Stroke group; G3: Stroke + MSC group; G4: Stroke + exercise group; G5: Stroke + MSC + exercise group; SD: Standard deviation.