Figure 8.

ADSC cytokine release in the spheroid and 2D monolayer culture supernatants. The spheroid and 2D monolayer cultures were incubated for 48 h, and the supernatants were collected and measured 24 h after the culture medium was changed. To estimate the number of cells in the spheroid and monolayer culture cells, the number of cells was measured by comparing the amount of luminescence by the Cell Titer-Glo 3D Cell Viability Assay. The number of cells was used to calculate the amount of cytokines produced per cell after 24 h. When a measured value below the detection limit was obtained, the detection limit was indicated in the graph. In addition, a statistical test was performed using the detection limit as the measured value. * indicates p < 0.05 using Student’s t-test.