Figure 9.

(A) Anti-oxidative potentials of αB∆21–28, ∆54–61 compared to αB-wt and αB∆54–61 in SI-treated ARPE-19 cells. ARPE-19 cells cultured on a 96-well plate were treated with SI and/or αB-crystallins for 24 h. The SI-induced reactive oxygen species (ROS) generation was measured by 2′,7′-Dichlorofluorescin diacetate (DCFH-DA) staining. The images were captured in EVOS FL Auto2 Imaging System (Thermo Fisher Scientific, Waltham, MA, USA) with 10× magnification. (B) The relative 2′,7′-dichlorofluorescein (DCF) intensity (green) was calculated using EVOS™ image analysis software (version 1.4.998.659). The data shown are an average of six analyses performed on images captured from different wells. The asterisks (***) represent a p-value < 0.005. The patterned bars represent chaperone proteins when used at 0.5 µM. The plain bars in chaperone protein-treated samples represent chaperone protein when tested at 1.0 µM.