Figure 1.

ZIP8 loss alters the intestinal microbial community and inferred functional capacity. 16S rRNA gene sequencing of WT and Zip8KO mice cecal microbial community. (A) Beta diversity of WT and Zip8KO mice, as determined by distance-based redundancy analysis (dbRDA) on sample-wise Bray–Curtis dissimilarity distances. (B) Differentially abundant ASVs as determined by DESeq2 using negative binomial generalized linear models for each taxa and Wald test for significances. (C) PCA plot of the inferred functional capacity of the cecal microbial communities from WT and Zip8KO mice. (D) Differentially abundant functional pathways, as determined by STAMP. n = 5/group.