Figure 4.

ZIP8-associated dysbiosis impairs pulmonary host defense. Mice recolonized with the cecal microbial community from WT and Zip8KO mice were infected with S. pneumoniae, and pulmonary host defense was assessed. (A) Weight change in recolonized mice post infection. (B) Representative histological staining of lung tissue, and (C) quantified inflammatory score. (D) Representative TUNEL staining of lung tissue and (E) quantitative mean pixel intensity. S. pneumoniae burden in the (F) lungs and (G) spleen. Bars represent the mean ± SEM and dots represent individual mice. p values are indicated in the figure and were determined by one-way ANOVA with Sidak’s multiple comparison test. n = 10/group, 5/experimental replicates.