Figure 6.

ZIP8-associated dysbiosis increases pulmonary inflammation. Mice recolonized with the cecal microbial community from WT and Zip8KO mice were infected with S. pneumoniae, and the number of pulmonary cytokine levels were assessed. (A) IL-6 levels in the BAL of recolonized mice post-infection. (B) CXCL1 levels in the BAL of recolonized mice post-infection. (C) TNF-α levels in the BAL of recolonized mice post-infection. (D) IFN-γ levels in the BAL of recolonized mice post-infection. Bars represent the mean ± SEM and dots represent individual mice. p values are indicated in the figure and were determined by one-way ANOVA with Sidak’s multiple comparison test. n = 10/group, 5/experimental replicate.