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. 2022 Jan 20;23(3):1114. doi: 10.3390/ijms23031114

Figure 2.

Figure 2

Coronal slice images including the VTA region around 3.2 mm posterior (upper panel). The dotted box marks the area of fluorescence micrographs of AAV-ChrimsonR expression (lower panel) from the corresponding image above; the scale bar is 100 µm. The red fluorescent neurons in the lower panel indicate neurons where ChrimsonR is expressed because mScarlet is expressed as a marker downstream of ChrimsonR. (a). Photograph of a section containing the VTA region of DAT-Cre mouse expressing ChrimsonR in the VTA. (b). Fluorescence micrographs show that only dopaminergic neurons in the VTA are specifically expressed. (c). Photographs depict the section containing the VTA region of a wild-type mouse expressing ChrimsonR in the VTA. (d). Fluorescence micrographs show that ChrimsonR is expressed not only in dopaminergic but also in non-dopaminergic neurons. (e). Photographs show the section containing the VTA region of a wild-type mouse as the control. (f). Fluorescence micrographs indicate that ChrimsonR is not expressed. Abbreviations: VTA, ventral tegmental area; AAV, adeno-associated virus.