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. 2022 Jan 18;23(3):1036. doi: 10.3390/ijms23031036

Figure 2.

Figure 2

miR-16-5p overexpression causes oxidative stress in cardiomyoblasts in vitro. (A) AOPP determination shows increased levels in AC16 cells after miR-16-5p overexpression (n = 6). (B) The levels of MDA content are unaltered in miR-16-5p-overexpressing AC16 cells (n = 6). (C) qPCR analysis shows reduced expression levels of the antioxidant genes CAT, SOD1, and GPX in AC16 cells after miR-16-5p overexpression (n = 6). (D) Representative western blots of CAT, SOD1, and GPx1 in AC16 cells after miR-16-5p overexpression. (E,F) Densitometry analysis of western blots show reduced levels of (E) CAT (n = 5) and (F) GPx1 (n = 4). * p < 0.05, ** p < 0.01, **** p < 0.0001, ns: nonsignificant.