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. 2022 Jan 20;23(3):1095. doi: 10.3390/ijms23031095

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of macrophage-derived exosomes on resistin expression in macrophages. (A), Representative Western blots for resistin and α-tubulin protein levels in macrophages subjected to basal glucose concentration (5.5 mM) stimulation for different periods of time. The macrophage-derived exosomes were extracted from macrophages under 25 mM glucose treatment for 1 h. (B), Quantitative analysis of resistin protein levels. The macrophage-derived exosomes were extracted from macrophages under 25 mM glucose treatment for 1 h. The values for stimulated macrophages have been normalized to the control cell values. (C), Upper panel, representative Western blots for resistin and α-tubulin protein levels in macrophages subjected to basal glucose concentration (5.5 mM) stimulation for different treatments. The macrophage-derived exosomes were extracted from macrophages under 25 mM glucose treatment for 1 h. Lower panel, quantitative analysis of resistin protein levels. The values for the stimulated macrophages have been normalized to the control cell values. (D), Quantitative real-time PCR analysis of resistin mRNA levels. The values for the treated macrophages are expressed as a ratio of the normalized values for resistin mRNA in the control cells. MALAT1 siRNA and the overexpression of miR-150-5p significantly reduced resistin expression induced by macrophage-derived exosomes from the basal glucose concentration (5.5 mM) treatment. Scramble siRNA was the control siRNA. The macrophage-derived exosomes were extracted from macrophages under 25 mM glucose treatment for 1 h. * p < 0.01 vs. control. ** p < 0.05 vs. control. n = 4 per group.

Effect of macrophage-derived exosomes on resistin expression in macrophages. (A), Representative Western blots for resistin and α-tubulin protein levels in macrophages subjected to basal glucose concentration (5.5 mM) stimulation for different periods of time. The macrophage-derived exosomes were extracted from macrophages under 25 mM glucose treatment for 1 h. (B), Quantitative analysis of resistin protein levels. The macrophage-derived exosomes were extracted from macrophages under 25 mM glucose treatment for 1 h. The values for stimulated macrophages have been normalized to the control cell values. (C), Upper panel, representative Western blots for resistin and α-tubulin protein levels in macrophages subjected to basal glucose concentration (5.5 mM) stimulation for different treatments. The macrophage-derived exosomes were extracted from macrophages under 25 mM glucose treatment for 1 h. Lower panel, quantitative analysis of resistin protein levels. The values for the stimulated macrophages have been normalized to the control cell values. (D), Quantitative real-time PCR analysis of resistin mRNA levels. The values for the treated macrophages are expressed as a ratio of the normalized values for resistin mRNA in the control cells. MALAT1 siRNA and the overexpression of miR-150-5p significantly reduced resistin expression induced by macrophage-derived exosomes from the basal glucose concentration (5.5 mM) treatment. Scramble siRNA was the control siRNA. The macrophage-derived exosomes were extracted from macrophages under 25 mM glucose treatment for 1 h. * p < 0.01 vs. control. ** p < 0.05 vs. control. n = 4 per group.