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. 2022 Jan 20;23(3):1123. doi: 10.3390/ijms23031123

Figure 2.

Figure 2

EN1 knockdown restrains the proliferation and migration of glioma cells. (A) Assessment of knockdown efficiency for EN1 with immunoblotting, and confirmation of EN1 knockout in U251 cells with immunoblotting and genome sequencing. Cell proliferation was assessed using CellTiter-Glo assays (B), 5-ethynyl-2′-deoxyuridine (Edu) assays (C), and colony formation assays (D) (● for shcon, ■ for shEN1-3898, ▲ for shEN1-3899, ◆ for shEN1-3901). (E) Cell migration was assessed with a wound-healing assay. (F) Quantification of wound-healing assay. (G) Survival curves for mice bearing xenograft tumors derived from shRNA-EN1 cells or scramble control cells. (H) Cell proliferation was assessed by CellTiter-Glo and colony formation assays in U-87 MG cells after EN1 overexpression. Scale bar: 100 μm. Data are presented as the mean ± SEM from three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 according to Student’s t-test.