Table 2.

Effect of 17β-estradiol (E2) and Parabens on Estrogen and Progesterone Receptors Gene and Protein Expression [52].

	MCF-7
	ERα				PR
	mRNA		Protein		mRNA		Protein
	6 h	24 h	48 h	72 h	6 h	24 h	48 h	72 h
MP	NC	↑	↑	↑	NC	NC	↑	↑
BP	NC	↑	↑	NC	NC	↑	NC	NC
PP	NC	↑	↑	NC	NC	↑	↑	↑
E2	NC	↑	↑	↑	↑	↑	↑	↑
	MCF-10A
	ERα				PR
	mRNA		Protein		mRNA		Protein
	6 h	24 h	48 h	72 h	6 h	24 h	48 h	72 h
MP	↑	NC	↑	↑	NC	NC	NC	NC
BP	NC	NC	NC	NC	NC	↑	NC	NC
PP	↑	NC	NC	↑	NC	↑	NC	NC
E2	NC	↑	↑	↑	NC	NC	NC	NC
	MCF-7				MCF-10A
	ERβ				ERβ
	mRNA		Protein		mRNA		Protein
	6 h	24 h	48 h	72 h	6 h	24 h	48 h	72 h
MP	NC	↑	↑	↑	NC	NC	NC	↑
BP	NC	↑	↑	NC	NC	NC	NC	↑
PP	NC	↑	↑	NC	NC	NC	NC	↑
E2	NC	↑	↑	↑	NC	NC	NC	NC

MCF-7 and MCF-10 A cells were exposed to parabens (20 nM) or E2 (10 nM); Gene expression was evaluated at 6 and 24 hours post treatment. Protein expression was evaluated at 48 and 72 hours post treatment. Up arrow: Upregulation; NC: No change.