Figure 2.

Effects of FFAs and Que treatment on the expression of ER stress marker. HepG2 cells were incubated in low glucose (LG) or in high glucose (HG) medium, in the presence or in the absence of 0.75 mM FFAs and 5µM Que for 24 h. Total proteins were extracted from the cells and separated by SDS/PAGE. After incubation with antibodies against XBP-1 or P-eIF2α, the content of XBP-1 and P-eIF2α was quantified by densitometric analysis and expressed as a percentage with respect to control cells (LG). Values are means ± S.D. Results are representative of three different experiments. * p < 0.05 versus control (LG); ** p < 0.01 versus control (LG).