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. 2022 Jan 19;23(3):1083. doi: 10.3390/ijms23031083

Figure 4.

Figure 4

Abrogation of endotoxin-enhanced mechanical ventilation-mediated mitochondrial biogenesis in HIF-1α-deficient mice. (A) SDH (complex II)) and (B) COX (complex IV) were from the diaphragms of nonventilated control mice and mice ventilated at a tidal volume of 10 mL/kg for 8 h with or without LPS administration (n = 5 per group). Western blots were performed using antibodies that recognize mitochondrial cytochrome C (C), PGC-1α (D), and GAPDH expression from the diaphragms of nonventilated control mice and mice ventilated at a tidal volume of 10 mL/kg for 8 h with or without LPS administration (n = 5 per group). Arbitrary units were expressed as relative mitochondrial cytochrome C and PGC-1α activation (n = 5 per group). * p < 0.05 versus the nonventilated control mice with LPS treatment; † p < 0.05 versus all other groups. COX = cytochrome-c oxidase; Mito-Cyt C = mitochondrial cytochrome C; PGC-1α = peroxisome proliferator activated receptor-γ coactivator; SDH = succinate dehydrogenase.