Figure 4.

Cellular proteome remodeling in ΔctaA mutant compared to its parental B. cereus AH187 strain (WT) at early exponential (EE), late exponential (LE), and stationary (S) growth phases. (a) Principal component analysis (PCA) showing reproducibility of WT and ΔctaA biological replicates and the dynamics of WT and ΔctaA cellular proteomes described by the first two components, PC1 and PC2. PC1 and PC2 explained 47.9% and 14.4% of total data variability, respectively. Replicates in each condition were plotted as a function of their PC1 and PC2 values. (b) Heat map of the 58 differentially-accumulated proteins (DAPs) showing their abundance changes (log₂ fold-change) in each growth phase. DAPs are indicated by their UniProt ID. Ascending hierarchical classification was determined based on Euclidean distance. The color code is as follows: red for down-DAPs, and blue for up-DAPS.