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(a,b) Calcein release assay for cholesterol-free L-aC-PC10 and L-aC-PC25 liposomes and relevant control (L) and cholesterol-containing L-Chol-aC-PC10 and L-Chol control in (a) PBS and (b) 50% human plasma (HP). Mean ± SD are reported. n = 3. (c) FRET assay utilizing the TMB-PC and BCHB-PC pair of lipid bilayer probes in L-aC-PC10, L-aC-PC25, and control (L) liposomes in PBS and 50% HP. n = 2. Mean ± SE are reported. For sample designations, see Table 1.
