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. 2022 Jan 18;23(3):1034. doi: 10.3390/ijms23031034

Figure 5.

Figure 5

Tubulin polymerization assay. Effect of various liposomal samples on tubulin aggregation at 34 μM in tubulin polymerization buffer at 37 °C: 1, paclitaxel, 3 μM, polymerization enhancer control (green triangles); 2, Tris-HCl, pH 8.25, buffer (black circles); 3, L, 0.4 mM total lipids (negative control; gray reversed triangles); 4, aC-PC, 40 μM (orange filled circles); 5, L-aC-PC25 (100 μM aC-PC; red filled squares); 6, L-aC-PC10 (equivalent to 40 μM aC-PC; blue filled squares); 7, L-aC-PC25 (100 μM aC-PC) hydrolyzed to aC (red empty squares); 8, L-aC-PC10 (40 μM aC-PC) hydrolyzed to aC (blue empty squares); 9, aC, 4 μM (orange empty circles). Mean ± SE are reported. n = 3. For sample designations, see Table 1.