Figure 7.

Densitometric analysis of the expression of neurotrophic factor, BDNF and anti-apoptotic factor Bcl-xL normalized to GAPDH: (A) Representative image of Western blot assay showing the expression of BDNF (14 kDa) and GAPDH (37 kDa); (B) BDNF level was reduced in the VEH animals compared to the WT, with a significance of p = 0.0019. The treatment significantly increased the expression of BDNF compared to the VEH (p < 0.0001) and the WT groups (p = 0.0049); (C) A representative image of Bcl-xL (30 kDa) and GAPDH (37 kDa) Western blot; (D) The anti-apoptotic factor Bcl-xL was reduced in the VEH group compared to the WT, with a significance of p = 0.0176. Pharmacological treatment significantly increased Bcl-xL protein level when compared to either the VEH (p < 0.0001) or the WT (p = 0.0001) groups. The same number of animals (n = 6 WT, 10 VEH, 12 TREATED) used for AMPK analysis was also used for the BDNF and Bc-xL studies. Results were analyzed by one-way ANOVA, followed by Tukey’s multiple comparisons test. Data are shown as mean ± SEM. * p < 0.05; ** p < 0.005; *** p < 0.0005; and **** p < 0.0001. Full length blots are shown in Supplementary Materials in Supplementary Data 2 Figure S9.