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Western blot of sperm homogenates from cattle, buffalo and sheep samples. Immunocomplexes obtained using the EpiGentek A62134 antibody against ATP12A and an anti-rabbit secondary antibody (Merck/MilliporeSigma) conjugated to peroxidase were detected at about 100 kDa using the ECL plus reagent (GE Healthcare Life Sciences; see Materials and Methods for details).
