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ATP12A and PNA localization in sperm cells from cattle. Representative fluorescence microscope images showing immunodetection of ATP12A (green) and TRITC-conjugated PNA (red) in frozen/thawed sperm cells. The secondary antibody for ATP12A was conjugated to Alexa Fluor 488. Nuclei were counterstained with DAPI (blue). Insets in the four panels represent 4× magnification of the same representative sperm cell as indicated by the white arrows. Scale bar: 10 μm.
