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ATP1B1 protein expression in sperm cells from cattle, buffalo and sheep. Representative fluorescence microscope images showing immunodetection of ATP1B1 (green) in frozen/thawed sperm cells. The secondary antibody was conjugated to Alexa Fluor 488. Nuclei were counterstained with DAPI (blue). Insets in the merge panels represent 3× magnification of representative sperm cells indicated by white arrows. Scale bar: 20 μm.
