Skip to main content
. 2022 Jan 18;23(3):1030. doi: 10.3390/ijms23031030

Figure 2.

Figure 2

Identification of GmLecRlk-ox transgenic soybean. (A) Physical maps of the regions between left border (LB) and right border (RB); (B) gel image of PCR products obtained with primer sets for T-DNA regions of the vector. M: DL2000 marker. 1–6: positive plants 7: negative control (ddH2O). 8: DNA of WT soybean plants. 9: plasmid of the pCAMBIA3300-GmLecRlk vector. (C) GmLecRlk gene expression level in transgenic and WT soybean by qRT-PCR. Significant differences were analyzed based on the results of three biological replications (Student’s t test: ** p < 0.01). Bars indicate the standard error of the means (s.e.m). (D) Detection of the selectable marker gene bar by test strip. Blue arrow: control. Red arrow: bar is positive. (E) GmLecRlk-ox and WT soybean were screened by smearing 180 mg/L PPT on the initial leaves.