Fig. 4.

Relative abundance of lipoxygenase cascade products in tobacco plant extracts after incubation with linoleic and α-linolenic acids: plants infected with the coronafacic acid-deficient Pectobacterium atrosepticum mutant (latent infection) (white boxplots); plants infected with the wild-type P. atrosepticum (typical infection) (grey boxplots). The abundance of oxylipins in the incubated extracts of control non-infected plants was taken as 1 (horizontal dashed line). The relative abundance of oxylipins was measured by integration of the total ion current GC-MS chromatograms. The content of oxylipins was normalized to the internal standard, margaric acid. Since JA (jasmonic acid) was not detected in the incubated extracts of control plants (as well as plants with latent infection), the minimal detectable peak area was assigned to JA in the extracts of control plants (and plants with latent infection) in order to calculate the relative content of JA in the extracts of plants with typical infection. The boxplots’ upper and lower whiskers extend from the fourth and second quartiles, respectively, to the extreme values no further than 1.5× interquartile range. Asterisks (*) show the significance of the difference in relative content between the experimental groups (Mann–Whitney two-sided test, n = 4, P < 0.05). 9-HOT, (9S,10E,12Z,15Z)-9-hydroxy-10,12,15-octadecatrienoic acid; 9-HOD, (9S,10E,12Z)-9-hydroxy-10,12-octadecadienoic acid; 9-oxo-9:0, 9-oxononanoic acid. The biosynthetic branch for a particular compound is given in parentheses.