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. 2021 Aug 21;129(3):271–286. doi: 10.1093/aob/mcab108

Fig. 8.

Fig. 8.

The total ion current GC-MS chromatograms of products (Me/TMS with preliminary NaBH4 reduction) of incubation of 9-HPOD (A), 9-HPOT (B), 13-HPOD (C), and 13-HPOT (D) with CYP74C43. 1a, 9-Hydroxynonanoic acid; 7, 9,10-epoxy-11-hydroxy-12-octadecenoic acid; 8, 9,10-epoxy-11-hydroxy-12,15-octadecadienoic acid; 10a, (9Z)-12-hydroxy-9-dodecenoic acid; 11a, (10E)-12-hydroxy-9-dodecenoic acid; 12, 11-hydroxy-12,13-epoxy-9-octadecenoic acid; 13, 11-hydroxy-12,13-epoxy-9,15-octadecadienoic acid. Incubation, extraction, derivatization and analysis procedures are described in the Materials and Methods. The structural formulae of the products are presented in Figs 3 and 9. 13(S)-HOT, (9Z,11E,13S,15Z)-13-hydroxy-9,11,15-octadecatrienoic acid; 13(S)-HOD, (9Z,11E,13S)-13-hydroxy-9,11-octadecadienoic acid; 9(S)-HOT, (9S,10E,12Z,15Z)-9-hydroxy-10,12,15-octadecatrienoic acid; 9(S)-HOD, (9S,10E,12Z)-9-hydroxy-10,12-octadecadienoic acid.