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. 2022 Jan 19;23(3):1063. doi: 10.3390/ijms23031063

Figure 3.

Figure 3

Mortalin knockdown suppresses TT cell growth in three-dimensional cultures. (A) Microscopy images of TT cells stably infected with doxycycline (dox)-inducible pTRIPZ virus that expresses shRNA targeting mortalin mRNA (dox-shMmir) or with the control pTRIPZ virus. Cells were treated with 0.5 ug/mL doxycycline (dox) for 9 days in the Matrigel culture. Expression of red fluorescence protein is a visual marking of dox-induced Tet-On activation. Scale bar = 100 µm. (B) Microscopy images of TT-dox-shMmir and TT-pTRIPZ cells seeded in 96-well spheroid culture plates at indicated cell density and treated with 0.5 ug/mL doxycycline for 6, 9, and 12 days. Scale bar = 100 µm. (C) Western blotting of total lysates of cells described in (A). β-actin is the control for equal loading. Microscope images and blots are representative of two independent experiments.