Figure 5.

Soluble ENG-stimulated microglia induces a hyper-angiogenic phenotype of endothelial cells. (A) BV2-sENG-CM induced EC migration in a scratch-wound assay. The rate of EC migration in each treatment was quantified by measuring the distance between the edges of scratched area at 10 h compared to 0 h after the scratch. n = 3; data are presented as mean ± SEM; * p < 0.05, ** p < 0.01; scale bar: 400 μm. (B) BV2-sENG-CM increased endothelial tube formation. The tube length, branch count, and loop count were automatically and blindly measured using Ibidi software. Three independent experiments were performed, and representative images are shown. n = 3; data are presented as mean ± SEM; * p < 0.05, ** p < 0.01, Student’s t-test; scale bar: 500 μm.