Figure 3.

Western diet (WD) and carbon tetrachloride (CCl4) treatment promote HCC formation in the mouse. (A) Schematic diagram of establishing a mouse HCC process. (B,C) Observation of appearance and body shape of mice in HCC and the range of weight change in time course. (D) Representative liver tissue macroscopic images and WD/CCl4 group indicate the number of red arrow numbers. (E) Liver tissue section of the WD/CCl4 group showed ki67 positive immunostaining. Scale bar, 2mm, and 40μm indicated low and high magnificently. (F) Masson trichrome stained livers presence of hepatic fibrosis and steatohepatitis. Scale bar, 40 μm. (G) Col3a1 gene expression elevated in WD/CCl4 treated group. Data per group are expressed as mean ± SEM calculated from six to seven mice. * p-value < 0.05; *** p-value < 0.0001 between groups.