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. 2022 Jan 24;23(3):1312. doi: 10.3390/ijms23031312

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Differentiation toward small intestinal epithelium (a) Intracellular localization of small intestinal epithelial cell markers in SIEC-iPSCs cultured either on Geltrex or laminin 511. Caco-2 cells grown on Geltrex served as a control. Enterocyte marker proteins PEPT1 (green) and villin (green), and enteroendocrine cell marker chromogranin A (CGA, green) were visualized with indirect immunofluorescence and confocal microscopy. In samples stained with Chromogranin A, the arrowhead in Geltrex, and arrow in laminin iPSC-SIEC samples point to extracellular matrix. Number of biological replicates n = 4, number of technical replicates n = 2. Scalebar 20 µm. (b) The expression of enterocyte marker genes PEPT1, villin and CYP3A4 was analyzed with qRT-PCR. The expression of iPSC-SIECs were calibrated against iPSC-SIEC cultured on Geltrex. Number of biological replicates n = 3, number of technical replicates in every biological replicate n = 3. Data expressed as mean ± SD. The statistical significance: *** (p ≤ 0.001).