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. 2022 Feb 2;23(3):1732. doi: 10.3390/ijms23031732

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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C7 immunostaining of RDEB-KC and skin equivalents. (A) C7-immunostaining (green) on RTM-transduced RDEB patient cells showed re-expression of C7. Cell nuclei were counterstained using DAPI (blue). (B) Correct localization of C7 at the BMZ was confirmed in cryosections of skin equivalents generated from RTMeA-transduced and mock-transduced (empty plasmid) patient KCs and in human wild-type (wt) skin. E = epidermis; D = dermis; arrows mark the dermal-epidermal junction. Scale bars: 50 μm.