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. 2022 Jan 29;23(3):1575. doi: 10.3390/ijms23031575

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Venn diagram showing the number of metabolites identified in serum samples of dogs infected with Babesia canis and healthy dogs. The untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics approach identified 64 metabolites, the targeted LC-MS metabolomics approach (Biocrates analysis) identified 205 metabolites, and 26 metabolites were identified by gas chromatography-mass spectrometry (GC-MS) metabolomics approach. A total of 295 metabolites were detected using all three platforms.