Figure 2.

Effect of PWBPH on transformation from fast-twitch to slow-twitch muscle fiber type. (A) The protein levels of fast MyHC and slow MyHC in QF muscle of the control, PWBPH 250 mg/kg, and Leu 500 mg/kg groups were determined by Western blotting and (B) the band intensities were measured using ImageJ software. (C–G) qRT-PCR analysis of the mRNA levels of slow-twitch muscle fiber-related genes (MyHC1, Tnni1, and Tnnc1) and fast-twitch muscle fiber-related genes (MyHC2b and MyHC2x). * p < 0.05 vs. control group. (H) Histological images of the QF muscle stained with slow-twitch and fast-twitch MyHC antibodies are shown (200× magnification).