Structure, GO analysis, and sequence alignment of ICE genes in plants. (A) 3D prediction of AtICE1 secondary structure, showing different domains denoted by different colors. (B) Gene ontology (GO) analysis of ICE1 in Arabidopsis. (C) A schematic presentation of AtICE1, depicting conserved binding domains and motifs. (D) Multiple alignments of ICE amino acid. Different color schemes in the background show conserved amino acid sequences within the conserved DNA-binding domains, the S-rich region, bHLH domain, the ZIP region, and the SUMO-conjugated motif in the ICE1 proteins. Shown sequences have been characterized from AtICE1 (A. thaliana), SlICE1 (S. lycopersicum), PtICE1 (P. trifoliata), PmICE1 (P. mume), VvICE1 (V. vinera), and CsICE1 (C. sinensis).