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Far-UV CD spectra of WT-RMPK (○) and of the RMPK mutants: E117K (□), K114Q (△), T113L/E117K (●), T113L/K114Q/E117K (■), T120L (▽), K114Q/E117K/T120L (▲) and T113L/K114Q/E117K/T120L (▼). The spectra were obtained in mixtures that contained each pyruvate kinase at a concentration of 100 μg/mL in 25 mM phosphate buffer pH 7.0 at 25 °C in cell pathway of 0.1 cm. CD is expressed as θMRW (mean residual weight ellipticity).
