Table 3.
List of DNA templates and primer sequences used in PCR.
| Name | Template DNA | Primer Sequence 5′→3′ |
|---|---|---|
| K114Q | WT-RMPK | FW_CTGGACACTCAGGGACCCGAG Rv_CTCGGGTCCCTGAGTGTCCAG |
| T120L | WT-RMPK | Fw_ATCCGGCTAGGCCTCATCAAGGGC Rv_GCCCTTGATGAGGCCTAGCCGGAT |
| T113L/E117K | E117K mutant 1 | Fw_CTGGACCTTAAGGGACCCAAGATCCGGACG Rv_AGTCCGGATCTTGGGTCCCTTAAGGTCCAG |
| T113L/K114Q/E117K | E117K mutant 1 | Fw_CTCTGGACCTGCAGGGACCC Rv_GGGTCCCTGCAGGTCCAGAG |
| K114Q/E117K/T120L | K114Q mutant | Fw_GGACCCAAGATCCGGCTAGGCCTCATCAAGGGC Rv_GCCCTTGATGAGGCCTAGCCGGATCTTGGGTCC |
| T113L/K114K/E117K/ T120L |
T113L/K114Q/E117K mutant |
|
| External (pTRC99A) | Fw_TAATCATCCGGCTCGTATAATGTG Rv_GGCTGAAAATCTTCTCTCATCCGC |
|
1 Reference [20].