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. 2022 Jan 24;23(3):1275. doi: 10.3390/ijms23031275

Figure 6.

Figure 6

Flow cytometry analysis of CD24 and CD13 in miR-21 KO cell lines. (A–C) Expression of CD13 and CD24 in the Panc-1 KOs: (A) Panc-1 vector alone (control cells); (B) miR-21 KO2; (C) miR-21 KO4. Expression levels of both CD13 and CD24 were significantly reduced in Panc-1 miR-21 KO2 and KO4, compared with the Panc-1 control cells. (D–F): Expression of CD13 and CD24 in MiaPaCa-2 KOs: (D) MiaPaCa-2 vector alone (control cells); (E) miR-21 KO2; (F) miR-21 KO4. Expression levels of CD24 were significantly increased in MiaPaCa-2 control cells, while CD13 was significantly reduced not only in MiaPaCa-2 control cells but also in MiaPaCa-2 miR-21 KO2 and miR-21 KO4. APC-Alexa 700 symbolizes the CD24 marker, while PE stands for CD13. Numbers in the gated areas mark the percentages of cells that were positive for this specific marker.