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. 2022 Feb 2;23(3):1724. doi: 10.3390/ijms23031724
AgTx2 agitoxin 2
GFP green fluorescent protein
MgTx margatoxin
HgTx1 hongotoxin 1
ChTx charybdotoxin
ScyTx scyllatoxin
TEA tetraethylammonium
IC50 a concentration of a competing ligand that displaces 50% of the fluorescent probe from the complex with the receptor
Kd dissociation constant of GFP–MgTx
Kap apparent dissociation constant of non-labeled ligand determined in the competitive binding experiment
Iav average fluorescence intensity of GFP–MgTx bound at a surface of a spheroplast expressing KcsA–Kv1.3
Iavs Iav value at saturated binding of GFP–MgTx
Im Iav value at zero concentration of a competing ligand in the competitive binding experiment