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. 2022 Jan 29;23(3):1572. doi: 10.3390/ijms23031572

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Transactivation activity assay of AtC3H12 in yeast. (a) Schematic map of the GAL4 BD-fusion vector for transactivation activity assay in yeast. (b) Schematic maps of full-length ORF, N197, and C207 of AtC3H12 for transactivation activity assay. (c) Quantitative β-galactosidase ONPG assay. β-Galactosidase activities were measured to quantify the transactivation activities. Error bars display standard deviation (n = 3). Different letters display significant differences (p < 0.05). (d) Yeast growth assay. Yeast transformants were grown on SD media lacking Trp and Ura (SD-Trp/-Ura). In (c,d), empty pBD-GAL4 vector was used for a negative control. NC, negative control.