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. 2022 Jan 27;23(3):1475. doi: 10.3390/ijms23031475

Figure 7.

Figure 7

The BMP receptor inhibitor LDN-212854 suppresses the angiogenesis of HCC xenografts through repression of ID1 in vivo. (A) Effect of LDN-212854 on the growth of Huh7 and MT cells. Mice were sacrificed after being injected with PBS or 6 mg/kg LDN-212854 twice daily for 10 days (Huh7 xenograft tumors) or 14 days (MT xenograft tumors). (B) Xenograft tumors of Huh7 and MT cells treated with PBS (n = 3) or LDN-212854 (n = 3). (C,D) Western blot analysis of ID1, HIF-1α, VEGFA, and VEGFR2 expression in xenograft tumors derived from Huh7 and MT cells. β-Actin was used as the reference for quantifying protein expression. (E) Representative IHC staining images of HIF-1α and VEGFR2 expression in Huh7 and MT cell xenografts. The error bars represent the SD from at least three independent biological replicates. Student’s t test was used to calculate p values, represented as * p < 0.05; ** p < 0.01.